The preferential interaction theory has been successfully applied to the description of salt effects in HIC retention. Fausnaugh and Regnier studied the HIC adsorption of several proteins in the presence of different types of salts and found that the solvophobic theory alone could not adequately explain retention differences. The solvophobic theory, based on the association and solvation of the participating species, describes retention in terms of the molal surface tension increment of the salt. There have been considerable efforts toward theoretical understanding of the mechanism of protein retention on hydrophobic chromatography surfaces. Design efforts would be greatly aided by a model that enables the reduction of the design space based on a fundamental understanding of the thermodynamics of HIC retention. Process design and optimization is usually achieved through exhaustive experimentation, and substantial work has been conducted to develop high-throughput experimental methods to facilitate the screening of a large process design space. informnikolase.live/acnt?_=1628123276660
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